How to Read a Methylene Blue Certificate of Analysis (COA)
Mark Kemp · 22 June 2026
What a methylene blue COA shows, the lines that matter, and how lot-level Eurofins testing covers every bottle.
A methylene blue Certificate of Analysis (COA) is a test report showing what an independent laboratory measured; for a consumer product, the safety-critical part is the heavy-metal screen, which is governed by the raw methylene blue lot rather than the bottling batch. Blu Brain's certificate is issued by Eurofins, a UKAS-accredited laboratory. It reports lead, cadmium, arsenic, and mercury individually by ICP-MS for the methylene blue lot from which every bottle is filled.
| Heavy metal | Result (mg/kg) | Method |
|---|---|---|
| Lead (Pb) | <0.005 | ICP-MS |
| Arsenic (As) | <0.002 | ICP-MS |
| Cadmium (Cd) | 0.007 | ICP-MS |
| Mercury (Hg) | 0.033 | ICP-MS |
Methylene blue lot MB25003 · Eurofins (UKAS testing lab 0342) · sample 400-2025-00215654.
What a Certificate of Analysis is and why it matters
A COA is a formal test report recording what a laboratory measured — the analytes tested, the method used, the numeric results, and who carried out the testing — as objective evidence rather than an in-house assurance. For methylene blue sold for consumer use, the part that matters most is the heavy-metal screen, because heavy-metal content is the main quality risk with cheaper, industrial-grade material. Crucially, heavy metals originate in the raw methylene blue powder, not the bottling — so the meaningful unit to test is the methylene blue lot. One screen governs every bottle filled from it.
The lines that matter — accredited lab and heavy-metal screen
Check four things. The testing laboratory and its accreditation — an independent, accredited lab (Blu Brain uses Eurofins, UKAS testing lab 0342) carries far more weight than a self-declared figure. The method — heavy metals should be measured by a quantitative technique such as ICP-MS, not estimated. The results — arsenic, cadmium, lead and mercury reported individually as numeric values, not a single word such as "Pass" or "Conforms", and read against a recognised limit framework (USP <232> / ICH Q3D). And traceability — the certificate should identify the material it covers — here, the methylene blue lot — so it can be tied back to the product. The contaminant context is on the safety page.
How to verify Blu Brain's COA
Blu Brain's certificate covers methylene blue lot MB25003, tested by Eurofins under certificate code AR-25-UD-215027-01 (sample 400-2025-00215654), reported by a named analyst. Every bottle is filled from this lot, so the screen applies to the product regardless of the bottling batch. Your bottle's box shows a batch number (for example, 1040) — that is the fill-batch, drawn from lot MB25003, and the same certificate covers it. To verify: confirm the issuing laboratory is Eurofins, that the four heavy-metal results are present as numeric ICP-MS values, and that the lot and certificate references are stated. A certificate with no named laboratory, no lot reference, or only "Pass" entries is a red flag.